1TMH
MODULAR MUTAGENESIS OF A TIM-BARREL ENZYME: THE CRYSTAL STRUCTURE OF A CHIMERIC E. COLI TIM HAVING THE EIGHTH (BETA-ALPHA)-UNIT REPLACED BY THE EQUIVALENT UNIT OF CHICKEN TIM
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.640, 118.310, 58.980 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 35.100 - 2.800 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.310 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 * |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.112 * |
| Total number of observations | 38490 * |
| Number of reflections | 17854 * |
| Completeness [%] | 82.0 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 60 (%) | |
| 2 | 1 | reservoir | MES-NaOH | 200 (mM) | |
| 3 | 1 | reservoir | DTT | 1 (mM) | |
| 4 | 1 | reservoir | 1 (mM) | ||
| 5 | 1 | drop | DTT | 1 (mM) | |
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | drop | 1 (mM) |
