1TLU
Crystal Structure of Thermotoga maritima S-adenosylmethionine decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.994, 104.994, 69.695 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.470 - 1.550 |
| R-factor | 0.155 |
| Rwork | 0.152 |
| R-free | 0.18700 |
| Structure solution method | SINGLE WAVELENGTH |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.470 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 39077 | |
| <I/σ(I)> | 29.3 | 3.1 |
| Completeness [%] | 98.2 | 98.2 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.4 | ammonium formate, HEPES, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 295.4K |
