1TLT

Crystal Structure of a Putative Oxidoreductase (VIRULENCE FACTOR mviM HOMOLOG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-20
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 61 2 2
Unit cell lengths	96.856, 96.856, 380.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.910 - 2.700
R-factor	0.245
Rwork	0.245
R-free	0.29400
Structure solution method	SAD
Starting model (for MR)	Experimental electron density.
RMSD bond length	0.011
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	29879
<I/σ(I)>	25.75	3.47
Completeness [%]	99.6	99.9
Redundancy	13.31	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	1.4M Ammonium Sulfate, 0.1M Na Acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K