1TLJ

Crystal Structure of Conserved Protein of Unknown Function SSO0622 from Sulfolobus solfataricus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	SBC-3
Wavelength(s)	0.97927,0.97948, 0.96404
Spacegroup name	P 32 2 1
Unit cell lengths	92.114, 92.114, 110.907
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.2489
Rwork	0.223
R-free	0.27160
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.082	0.399
Number of reflections	25954
<I/σ(I)>	8.3	2.99
Completeness [%]	99.4	100
Redundancy	5.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	PEG 5000MME, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K