1TKY

Crystal structure of the editing domain of threonyl-tRNA synthetase complexed with seryl-3'-aminoadenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-24
Detector	MARRESEARCH
Wavelength(s)	0.9330
Spacegroup name	P 21 21 21
Unit cell lengths	43.563, 45.429, 116.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.520 - 1.480
R-factor	0.203
Rwork	0.201
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qf6
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.520	1.520
High resolution limit [Å]	1.470	1.470
Rmerge	0.071	0.228
Number of reflections	39904
Completeness [%]	98.8	93.4
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	PEG 4000, Tris.cl, magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K