1TKU

Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-21
Detector	MARRESEARCH
Wavelength(s)	1.542
Spacegroup name	P 1
Unit cell lengths	40.200, 47.900, 59.800
Unit cell angles	66.40, 72.30, 89.90

Refinement procedure

Resolution	20.000 - 1.660
Rwork	0.184
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g57
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.672
High resolution limit [Å]	1.660	1.660
Rmerge	0.032	0.174
Number of reflections	39461
<I/σ(I)>	22.2
Completeness [%]	77.3	28.7
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	Mes/NaOH, PEG8000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K