1TKO
Iron-oxo clusters biomineralizing on protein surfaces. Structural analysis of H.salinarum DpsA in its low and high iron states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.731 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 91.110, 91.110, 150.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
R-factor | 0.18396 |
Rwork | 0.178 |
R-free | 0.26281 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1moj |
RMSD bond length | 0.060 |
RMSD bond angle | 4.378 |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.093 |
Number of reflections | 15444 |
<I/σ(I)> | 9.75 |
Completeness [%] | 94.2 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG400, 1M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |