1TKJ

Streptomyces griseus aminopeptidase complexed with D-Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	95
Detector technology	CCD
Collection date	2001-09-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97930
Spacegroup name	P 41 21 2
Unit cell lengths	60.962, 60.962, 144.705
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.150
Rwork	0.117
R-free	0.15120
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.014
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.170
High resolution limit [Å]	1.150	1.150
Number of reflections	92150
<I/σ(I)>	20.1
Completeness [%]	94.2	63.4
Redundancy	8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K