1TKB

SPECIFICITY OF COENZYME BINDING IN THIAMIN DIPHOSPHATE DEPENDENT ENZYMES: CRYSTAL STRUCTURES OF YEAST TRANSKETOLASE IN COMPLEX WITH ANALOGS OF THIAMIN DIPHOSPHATE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	76.300, 113.300, 160.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.300
R-factor	0.185
Rwork	0.185
RMSD bond length	0.016
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.100 *
Total number of observations	97580 *
Number of reflections	44174 *
Completeness [%]	73.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	PEG6000	15 (%)
3	1	reservoir	glycylglycine	50 (mM)
4	1	reservoir	thiamine-PP	2 (mM)