1TK3

Crystal Structure Of Human Apo Dipeptidyl Peptidase IV/CD26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	119.264, 122.360, 129.823
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.680 - 2.000
R-factor	0.229
Rwork	0.229
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n1m
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNX (2000.1)
Refinement software	CNX (2000.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.680	2.130
High resolution limit [Å]	2.000	2.000
Number of reflections	116526
Completeness [%]	90.6	79.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 4000, SODIUM ACETATE, TRIS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K