1TK1
YEAST OXYGEN-DEPENDENT COPROPORPHYRINOGEN OXIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-13 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 70.850, 70.850, 114.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.760 - 1.900 |
| R-factor | 0.21713 |
| Rwork | 0.215 |
| R-free | 0.24907 |
| Structure solution method | SAD - CHROMIUM SULFUR ANOMALOUS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.851 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.585 |
| Number of reflections | 21772 | |
| <I/σ(I)> | 20 | 7 |
| Completeness [%] | 96.9 | 95.6 |
| Redundancy | 28.7 | 29.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 20% PEG 3000, 0.1 M HEPES, 0.2 M sodium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 7.50 |
