1TJW

Crystal Structure of T161D Duck Delta 2 Crystallin Mutant with bound argininosuccinate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	93.996, 98.806, 106.387
Unit cell angles	90.00, 101.34, 90.00

Refinement procedure

Resolution	41.760 - 2.000
R-factor	0.19
Rwork	0.190
R-free	0.22500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1hy1
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.800	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	128293
Completeness [%]	99.7	99.4
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	14% PEG2000 MME, 350 mM magnesium chloride, 100 mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K