1TJN
Crystal structure of hypothetical protein af0721 from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-22 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979374,0.979238,0.964108 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 102.460, 102.460, 59.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.460 - 2.010 |
| R-factor | 0.21952 |
| Rwork | 0.217 |
| R-free | 0.26006 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.299 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.010 |
| Rmerge | 0.060 | |
| Number of reflections | 10173 | |
| Completeness [%] | 85.2 | 82.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | 1.9 M AMMONIUM SULFATE, 0.1 M HEPES, 4% MPD, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
