1TJL
Crystal structure of transcription factor DksA from E. coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0, 1.28270, 1.28300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.319, 96.593, 117.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.950 - 2.000 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.400 |
| Number of reflections | 135624 | |
| <I/σ(I)> | 28 | 3.4 |
| Completeness [%] | 98.4 | 96.2 |
| Redundancy | 5.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | PEG4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
