1TJK
Crystal structure of the complex formed between group II phospholipase A2 with a designed pentapeptide, Phe- Leu- Ser- Thr- Lys at 1.2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-04-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8030 |
Spacegroup name | P 43 |
Unit cell lengths | 52.293, 52.293, 47.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.990 - 1.250 |
R-factor | 0.18454 |
Rwork | 0.184 |
R-free | 0.20273 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1skg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.725 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.280 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 35680 | |
Completeness [%] | 100.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Ammonium Sulphate, Polyethylene glycol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |