1TJ6
Crystal structure of the Xenopus tropicalis Spred1 EVH-1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.881, 38.267, 80.226 |
| Unit cell angles | 90.00, 95.58, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.650 |
| R-factor | 0.1539 |
| Rwork | 0.151 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1evh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | 0.211 |
| Number of reflections | 23273 | |
| <I/σ(I)> | 38.2 | 9.35 |
| Completeness [%] | 96.2 | 94.6 |
| Redundancy | 6.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 3350, potassium fluoride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
