1TIS

CRYSTAL STRUCTURE OF THYMIDYLATE SYNTHASE FROM T4 PHAGE

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	68.020, 68.020, 140.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.199
Rwork	0.199
RMSD bond length	0.016
RMSD bond angle	3.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.068 *	0.257 *
Total number of observations	30518 *
Number of reflections	6186 *	192 *
Completeness [%]	65.0 *	12 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	thymidylate synthase from phage T4	4.35 (mg/mL)
2	1	drop	phosphate	0.2 (M)
3	1	reservoir	ammonium sulfate	25-30 (%)