1TIO
HIGH PACKING DENSITY FORM OF BOVINE BETA-TRYPSIN IN CYCLOHEXANE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Collection date | 1996-04-15 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.540, 58.260, 67.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.930 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bty |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.000 * |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.000 |
| High resolution limit [Å] | 1.930 |
| Rmerge | 0.088 |
| Total number of observations | 34500 * |
| Number of reflections | 14880 |
| <I/σ(I)> | 12 |
| Completeness [%] | 88.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 * | 20 * | HANGING DROP METHOD: THE PROTEIN WAS DISSOLVED IN 10 MM HAC-NAAC BUFFER (PH 4.1) PRECIPITANT AND RESERVOIR SOLUTION WERE 0.5 M NA2SO4 AND 0.1 M SODIUM CACODYLATE-HCL BUFFER CONTAINING 15% (W/V) MGSO4 AND 1.5% (V/V) MPD AT PH 6.2., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | HAc-NaAc | 10 (mM) | |
| 3 | 1 | drop | benzamidine | 16 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | reservoir | sodium cacodylate-HCl | 0.1 (M) | |
| 6 | 1 | reservoir | 15 (%(w/v)) | ||
| 7 | 1 | reservoir | 0.5 (M) | ||
| 8 | 1 | reservoir | MPD | 1.5 (%(v/v)) |
