1THJ

CARBONIC ANHYDRASE FROM METHANOSARCINA

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Detector technology	IMAGE PLATE
Collection date	1995-07-03
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	71.840, 71.840, 333.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.234
Rwork	0.234
R-free	0.29500
RMSD bond length	0.013
RMSD bond angle	27.040 *
Data reduction software	DENZO
Data scaling software	Agrovata
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.800 *
Rmerge	0.064	0.324 *
Number of reflections	21401
Completeness [%]	95.1	82.9 *
Redundancy	3	2.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.0 (M)
2	1	1	PEG400	3 (%)
3	1	2	cacodylate	0.1 (M)
4	1	2		0.02 (%)