1TH8
Crystal Structures of the ADP and ATP bound forms of the Bacillus Anti-sigma factor SpoIIAB in complex with the Anti-anti-sigma SpoIIAA: inhibitory complex with ADP, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97950 |
Spacegroup name | H 3 2 |
Unit cell lengths | 153.646, 153.646, 97.517 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
Rwork | 0.214 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ID 1L0O pdb entry ID 1H4Y |
RMSD bond length | 0.006 |
RMSD bond angle | 1.199 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 19426 | |
<I/σ(I)> | 20.8 | 3.6 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295.5 | Sodium Malonate, HEPES, Jeffamine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |