1TGX
X-RAY STRUCTURE AT 1.55 A OF TOXIN GAMMA, A CARDIOTOXIN FROM NAJA NIGRICOLLIS VENOM. CRYSTAL PACKING REVEALS A MODEL FOR INSERTION INTO MEMBRANES
Experimental procedure
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.670, 40.710, 56.020 |
Unit cell angles | 90.00, 117.08, 90.00 |
Refinement procedure
Resolution | 5.000 - 1.550 |
R-factor | 0.172 |
Rwork | 0.172 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.044 * |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.300 * | |
High resolution limit [Å] | 1.550 * | 1.550 * |
Rmerge | 0.061 * | |
Total number of observations | 56659 * | |
Number of reflections | 17217 * | |
Completeness [%] | 100.0 * | 40 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4.5 * | macro seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | 2.2-2.4 (M) |