1TGX

X-RAY STRUCTURE AT 1.55 A OF TOXIN GAMMA, A CARDIOTOXIN FROM NAJA NIGRICOLLIS VENOM. CRYSTAL PACKING REVEALS A MODEL FOR INSERTION INTO MEMBRANES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	78.670, 40.710, 56.020
Unit cell angles	90.00, 117.08, 90.00

Refinement procedure

Resolution	5.000 - 1.550
R-factor	0.172
Rwork	0.172
RMSD bond length	0.009
RMSD bond angle	0.044 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.300 *
High resolution limit [Å]	1.550 *	1.550 *
Rmerge	0.061 *
Total number of observations	56659 *
Number of reflections	17217 *
Completeness [%]	100.0 *	40 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5 *		macro seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir		2.2-2.4 (M)