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1TGX

X-RAY STRUCTURE AT 1.55 A OF TOXIN GAMMA, A CARDIOTOXIN FROM NAJA NIGRICOLLIS VENOM. CRYSTAL PACKING REVEALS A MODEL FOR INSERTION INTO MEMBRANES

Experimental procedure
Spacegroup nameC 1 2 1
Unit cell lengths78.670, 40.710, 56.020
Unit cell angles90.00, 117.08, 90.00
Refinement procedure
Resolution5.000 - 1.550
R-factor0.172
Rwork0.172
RMSD bond length0.009
RMSD bond angle0.044

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Phasing softwareX-PLOR
Refinement softwarePROLSQ
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]2.300

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High resolution limit [Å]1.550

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1.550

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Rmerge0.061

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Total number of observations56659

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Number of reflections17217

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Completeness [%]100.0

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40

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Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

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4.5

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macro seeding

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein30 (mg/ml)
21reservoir2.2-2.4 (M)

229380

PDB entries from 2024-12-25

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