1TGR
Crystal Structure of mini-IGF-1(2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.860, 61.980, 70.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.420 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | SAD or SIRAS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.621 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.096 | 0.376 |
| Number of reflections | 23375 | |
| <I/σ(I)> | 16.7 | 4.6 |
| Completeness [%] | 99.6 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.65 | 289 | Citrate, ethanol, pH 6.65, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
