1TGM
Crystal structure of a complex formed between group II phospholipase A2 and aspirin at 1.86 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 53.314, 53.314, 48.453 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.860 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.22403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.524 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 11524 | |
| <I/σ(I)> | 6.5 | 2.5 |
| Completeness [%] | 98.5 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Polyethylene glycol, ammonium sulphate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
