1TGK

HUMAN TRANSFORMING GROWTH FACTOR BETA 3, CRYSTALLIZED FROM PEG 4000

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1992-12-23
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 61 2 2
Unit cell lengths	77.800, 77.800, 143.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.300
R-factor	0.228
Rwork	0.228
RMSD bond length	0.016
RMSD bond angle	1.920
Data reduction software	ROTAVATA-AGROVATA
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	3.300 *	3.300 *
Rmerge	0.132
Number of reflections	4232
Completeness [%]	100.0	100 *
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.4 *	20-22 *	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5-3 (mg/ml)
2	1	drop	acetic acid	5 (mM)
3	1	drop	PEG4000	15 (%(w/v))
4	1	drop	magnesium chloride	100 (mM)
5	1	drop	Tris-HCl	50 (mM)	pH8.4
6	1	reservoir	PEG4000	30 (%(w/v))
7	1	reservoir	magnesium chloride	200 (mM)
8	1	reservoir	Tris-HCl	100 (mM)	pH8.4