1TGJ
HUMAN TRANSFORMING GROWTH FACTOR-BETA 3, CRYSTALLIZED FROM DIOXANE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-12-16 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 49.300, 49.300, 78.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| Structure solution method | MR |
| Starting model (for MR) | TGF-BETA2 (SCHLUNEGGER & GRUETTER) |
| RMSD bond length | 0.070 |
| RMSD bond angle | 24.890 * |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 2.100 |
| High resolution limit [Å] | 2.000 * | 2.000 |
| Rmerge | 0.070 | 0.353 |
| Number of reflections | 6323 | |
| Completeness [%] | 76.0 | 37.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 15% (V/V) DIOXANE, 100 MM SODIUM ACETATE, PH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-6 (mg/ml) | |
| 2 | 1 | drop | acetic acid | 10 (mM) | |
| 3 | 1 | reservoir | dioxane | 15 (%(v/v)) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.0 |
