1TFY
How CCA is added to the 3' end of immature tRNA without the use of an oligonucleotide template
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.092, 84.023, 134.561 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 44.280 - 3.200 |
| R-factor | 0.23801 |
| Rwork | 0.235 |
| R-free | 0.29043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r89 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.098 | |
| Number of reflections | 38861 | |
| Completeness [%] | 99.0 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
