1TFP
TRANSTHYRETIN (FORMERLY KNOWN AS PREALBUMIN)
Experimental procedure
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 77.660, 77.660, 161.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.760 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.083 |
| Total number of observations | 22739 * |
| Number of reflections | 10781 |
| Completeness [%] | 78.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | sodium azide | 0.02 (%) | |
| 4 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
| 5 | 1 | reservoir | PEG8000 | 20 (%) |
