1TF9

Streptomyces griseus aminopeptidase complexed with P-Iodo-L-Phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	95
Detector technology	CCD
Collection date	2001-09-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	61.053, 61.053, 144.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.300
Rwork	0.122
R-free	0.17220
Structure solution method	Difference fourier from previously determined, related structure
RMSD bond length	0.012
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	63168
<I/σ(I)>	17.2
Completeness [%]	92.7	57.8
Redundancy	11	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K