1TDW
Crystal structure of double truncated human phenylalanine hydroxylase BH4-responsive PKU mutant A313T.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.411, 108.138, 124.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.100 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23700 |
| Structure solution method | Difference fourier |
| Starting model (for MR) | 1pah |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.118 | 0.612 |
| Number of reflections | 26608 | |
| <I/σ(I)> | 20 | 20 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 5-10% Ethylene Glycol, 20-40 mM Pipes, pH 6.8, 8-15% PEG 2000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
