1TC5

Structural Analysis of a probable eukaryotic D-amino acid tRNA deacylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.91840
Spacegroup name	P 2 2 21
Unit cell lengths	67.479, 67.465, 174.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.130 - 1.930
R-factor	0.20053
Rwork	0.199
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMAD model which crystallized in a different space group (p21)
RMSD bond length	0.012
RMSD bond angle	1.279
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.673	2.020
High resolution limit [Å]	1.930	1.930
Rmerge	0.058	0.238
Number of reflections	48177
<I/σ(I)>	10.2	2
Completeness [%]	79.4	28.6
Redundancy	3.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 200, MES, PEG3000, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K