1TAR

CRYSTALLINE MITOCHONDRIAL ASPARTATE AMINOTRANSFERASE EXISTS IN ONLY TWO CONFORMATIONS

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	57.400, 59.400, 65.500
Unit cell angles	83.10, 104.80, 83.30

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.194
RMSD bond length	0.014
RMSD bond angle	2.200
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.069 *
Total number of observations	44017 *
Number of reflections	36893 *
Completeness [%]	88.5 *	84.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	17-20 (%(w/v))
2	1	reservoir	ammonium acetate	0.5 (M)
3	1	reservoir	phosphate	20 (mM)	pH7.5