1T9R

Catalytic Domain Of Human Phosphodiesterase 5A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	93
Detector technology	CCD
Collection date	2002-12-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0, 1.282927, 1.283302, 1.169662
Spacegroup name	P 62
Unit cell lengths	96.411, 96.411, 79.026
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.510 - 2.100
R-factor	0.20359
Rwork	0.202
R-free	0.24125
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.421
Data scaling software	ELVES
Phasing software	CNX
Refinement software	REFMAC (5.1.25)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.510	2.240
High resolution limit [Å]	2.100	2.100
Rmerge	0.047
Number of reflections	23081
<I/σ(I)>	8.2
Completeness [%]	99.3	99.1
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	PEG 3000, sodium chloride, sodium phosphate-citrate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K