1T9K
X-ray crystal structure of aIF-2B alpha subunit-related translation initiation factor [Thermotoga maritima]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-17 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.885, 103.885, 259.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.330 - 2.600 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.093 | 0.415 |
| Number of reflections | 96655 | |
| <I/σ(I)> | 27.9 | 5.28 |
| Completeness [%] | 100 | |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | PEG 4000, Lithium Sulfate, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
