1T98
Crystal Structure of MukF(1-287)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1272, 0.9797 |
| Spacegroup name | P 61 |
| Unit cell lengths | 58.699, 58.699, 307.414 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.471 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 12713 | |
| <I/σ(I)> | 12.25 | 4.5 |
| Completeness [%] | 95.9 | 95.9 |
| Redundancy | 5.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | PEG 2K MME, calcium chloride, ammonium phosphate, bis-tris propane, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 8.50 |
