1T8S

Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9778
Spacegroup name	P 42 21 2
Unit cell lengths	246.240, 246.240, 111.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.750 - 2.600
R-factor	0.226
Rwork	0.226
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	100422
<I/σ(I)>	37.9	4
Completeness [%]	95.8	100
Redundancy	7.7	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	291	ammonium formate, sodium chloride, sodium citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K