1T7L
Crystal Structure of Cobalamin-Independent Methionine Synthase from T. maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.565, 158.763, 64.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data scaling software | XDS |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 112572 | |
| <I/σ(I)> | 13.77 | 4.35 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 7.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR BATCH, UNDER OIL | 4.6 | 295 | PEG 4000, sodium acetate, ammonium sulfate, pH 4.6, VAPOR BATCH, UNDER OIL, temperature 295.0K |
