1T71
Crystal structure of a novel phosphatase Mycoplasma pneumoniaefrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2003-06-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.896, 84.896, 162.726 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.100 |
| R-factor | 0.2151 |
| Rwork | 0.215 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 31951 | |
| Completeness [%] | 89.7 | 56.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Bis-Tris, AMS, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
