1T6Z
Crystal structure of riboflavin bound TM379
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.535, 81.943, 66.709 |
| Unit cell angles | 90.00, 116.96, 90.00 |
Refinement procedure
| Resolution | 19.370 - 2.400 |
| R-factor | 0.2251 |
| Rwork | 0.225 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 24508 | |
| Completeness [%] | 95.0 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, sodium citrate, glycerol, propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
