1T6N

Crystal structure of the N-terminal domain of human UAP56

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-10
Wavelength(s)	0.97921, 0.97923, 0.96417
Spacegroup name	P 1 21 1
Unit cell lengths	49.453, 47.284, 87.190
Unit cell angles	90.00, 98.23, 90.00

Refinement procedure

Resolution	30.000 - 1.940
R-factor	0.216
Rwork	0.216
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.340
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.060	0.450
Number of reflections	29132
<I/σ(I)>	25.5	5.4
Completeness [%]		98.7
Redundancy	5.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	sodium citrate, PEG MME 2000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K