1T6L

Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-23
Detector	BRANDEIS - B4
Wavelength(s)	0.9786, 0.9788, 0.9500
Spacegroup name	P 61 2 2
Unit cell lengths	53.930, 53.930, 340.067
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 1.850
R-factor	0.23831
Rwork	0.235
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.577
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.850	1.850
Number of reflections	26592
<I/σ(I)>	58.4	14.2
Completeness [%]	99.8	100
Redundancy	28.8	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	4.2	295	polyethylene glycol 4000, sodium acetate, sodium chloride, dithiothreitol , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K