1T6A
Crystal Structure of Protein of Unknown Function from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97952 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 77.307, 77.307, 200.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.050 |
| R-factor | 0.19618 |
| Rwork | 0.194 |
| R-free | 0.22462 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.088 | 0.432 |
| Number of reflections | 14757 | |
| <I/σ(I)> | 22.4 | 2.07 |
| Completeness [%] | 99.3 | 94.3 |
| Redundancy | 9.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | Bis-Tris-Propane, Sodium Nitrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
