1T62
Crystal structure of protein EF3133 from Enterococcus faecalis V583, Pfam DUF984
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-01-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0083 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.250, 88.250, 106.001 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.219 |
| Rwork | 0.215 |
| R-free | 0.26300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 9754 | |
| Completeness [%] | 98.4 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 2000, Succinic Acid, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
