1T5B
Structural genomics, A protein from Salmonella typhimurium similar to E. coli acyl carrier protein phosphodiesterase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-25 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.891, 107.915, 51.168 |
| Unit cell angles | 90.00, 111.45, 90.00 |
Refinement procedure
| Resolution | 30.550 - 1.400 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.038 | 0.165 |
| Number of reflections | 164570 | |
| <I/σ(I)> | 32.07 | 4.63 |
| Completeness [%] | 96.3 | |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 10% Glycerol, 20.5% PEG 4000, 0.065 M tris-sodium Citrate, 0.1 M NH4 acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
