1T57

Crystal Structure of the Conserved Protein MTH1675 from Methanobacterium thermoautotrophicum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-20
Detector	SBC-2
Wavelength(s)	0.9794, 0.9795, 0.956
Spacegroup name	P 21 21 2
Unit cell lengths	103.983, 69.398, 90.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.920 - 2.300
R-factor	0.259
Rwork	0.252
R-free	0.31800
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.800
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.538
Number of reflections	28562
<I/σ(I)>	8.8	1.8
Completeness [%]	95.7	93.6
Redundancy	5.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7	298	HEPES, magnesium acetate, PEG 3350, Glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K