1T4O
Crystal structure of rnt1p dsRBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-02 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.815, 68.312, 57.178 |
| Unit cell angles | 90.00, 121.91, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.500 |
| R-factor | 0.20582 |
| Rwork | 0.198 |
| R-free | 0.27637 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t4n |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.490 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 7943 | |
| Completeness [%] | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 0.2M lithium sulfate, 30% PEG 4000, 12% MPD, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.50 |
