1T4G
ATPase in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | ENRAF-NONIUS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 83.699, 83.699, 106.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.220 |
| Data reduction software | LSCALE |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 38530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M magnesium chloride, 0.1M Tris, 6% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
