1T49

Allosteric Inhibition of Protein Tyrosine Phosphatase 1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-08-16
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	88.394, 88.394, 104.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.2069
Rwork	0.205
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.361
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.670	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	36619
Completeness [%]	99.6	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	VAPOR DIFFUSION, HANGING DROP, temperature 277K