1T3S

Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9333
Spacegroup name	P 21 21 2
Unit cell lengths	74.066, 163.841, 34.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.650 - 2.300
R-factor	0.26222
Rwork	0.261
R-free	0.27728
Structure solution method	MAD
Starting model (for MR)	model built from earlier MAD data
RMSD bond length	0.008
RMSD bond angle	1.536
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.048	0.310
Number of reflections	18271
<I/σ(I)>	15.4	4.3
Completeness [%]		87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	292	PEG 20K, BICINE, SODIUM CHLORIDE, BETA-MERCAPTOETHANOL, MPD, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 292K