1T2T
Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.660, 64.890, 42.810 |
| Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.500 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1i3j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | |
| Number of reflections | 10240 | |
| Completeness [%] | 98.2 | 98.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | MES, glycerol, sodium formate, sodium chloride, PEG 3350, isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MES | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 1 | sodium formate | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | PEG | ||
| 6 | 1 | 1 | isopropanol |
