1T24
Plasmodium falciparum lactate dehydrogenase complexed with NAD+ and 4-hydroxy-1,2,5-oxadiazole-3-carboxylic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-01 |
Detector | MACSCIENCE |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 80.488, 85.914, 90.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.360 - 1.700 |
R-factor | 0.1437 |
Rwork | 0.142 |
R-free | 0.16668 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ldg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.544 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.360 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 33448 | |
<I/σ(I)> | 18 | 5.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | MPD, Hepes, Imidazole, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |